lin-45 Antibody

Code CSB-PA781400XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans lin-45 Polyclonal antibody
Uniprot No.
Target Names
lin-45
Alternative Names
lin-45 antibody; raf-1 antibody; Y73B6A.5Raf homolog serine/threonine-protein kinase antibody; EC 2.7.11.1 antibody; Abnormal cell lineage protein 45 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans lin-45 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein kinase that participates in the induction of vulva and has roles in fertility and viability. Acts downstream of the Ras protein let-60. Required for progression of developing oocytes through the pachytene stage. Plays a role in responses to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation. Positively regulates lifespan upstream of mek-2 and mpk-1.
Gene References into Functions
  1. Expression of activated LIN-45/Raf in head acetylcholine neurons is sufficient to cause an exaggerated waveform phenotype and hypersensitivity to the acetylcholinesterase inhibitor aldicarb. PMID: 29615470
  2. Genetic analysis indicates that lin-45 and protein kinase C (PKC)-1 act together to regulate nose touch response; these results functionally link two conserved signaling pathways in adult C. elegans neurons and define distinct roles for PKC genes in vivo. PMID: 21143768
  3. Connector/eNhancer of KSR promotes LIN-45 Raf activation at a step between the dephosphorylation of inhibitory sites in the regulatory domain and activating phosphorylation in the kinase domain PMID: 16085714
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
Database Links

STRING: 6239.Y73B6A.5a.2

UniGene: Cel.18190

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