KTI12 Antibody

Code CSB-PA330329XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KTI12 Polyclonal antibody
Uniprot No.
Target Names
KTI12
Alternative Names
KTI12 antibody; YKL110C antibody; YKL446 antibody; YKL500Protein KTI12 antibody; Gamma-toxin target protein 4 antibody; Killer toxin insensitivity protein 12 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KTI12 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Elongator-associated factor that is not a structural subunit but rather transiently contacts Elongator, presumably to promote its interaction with elongation competent pol II. Regulates Elongator complex gamma-toxin target (TOT) activity, resulting in G1 block by Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin), probably through regulation of the Elongator IKI3 subunit phosphorylation state. Required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.
Gene References into Functions
  1. The conserved functions of Arabidopsis DRL1 and yeast KTI12 deomnstrated by genetic complementation. PMID: 25518926
  2. close physical and functional relationship between Elongator and the highly conserved Kti12 protein PMID: 15772087
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
KTI12 family
Database Links

KEGG: sce:YKL110C

STRING: 4932.YKL110C

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