LAC1 Antibody

Code CSB-PA329818XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LAC1 Polyclonal antibody
Uniprot No.
Target Names
LAC1
Alternative Names
LAC1; DGT1; YKL008C; YKL156; Ceramide synthase LAC1; Very-long-chain ceramide synthase LAC1
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LAC1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the ceramide synthase complex that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward hexacosanoyl-CoA (C26:0-CoA). N-acylates sphinganine and phytosphingosine bases to form dihydroceramides and phytoceramides, respectively. Redundant with LAG1. Facilitates ER-to-Golgi transport of GPI-anchored proteins.
Gene References into Functions
  1. Lac1 and Lag1 are direct targets for CK2 and that phosphorylation at conserved positions within the C-terminal cytoplasmic domain of each protein is required for optimal CerS activity PMID: 25429105
  2. Data demonstrate that the pleiotropic drug resistance regulatory pathway regulates the transcription of multiple genes encoding steps in sphingolipid biosynthesis, including LAC1, but not LAG1. PMID: 15302821
  3. the topology of the Lag1p and Lac1p subunits of ceramide synthase was investigated PMID: 16756512
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Sphingosine N-acyltransferase family
Database Links

KEGG: sce:YKL008C

STRING: 4932.YKL008C

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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