Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LOH1 Polyclonal antibody

Uniprot No.

Q9LDF2

Target Names

LOH1

Alternative Names

LOH1; LAG1; At3g25540; MWL2.19; Ceramide synthase LOH1; CS1; CSII; Protein LONGEVITY ASSURANCE GENE ONE HOMOLOG 1; LAG One Homolog 1; LAG1 homolog 1; LAG1 longevity assurance homolog 1

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LOH1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential for plant growth, promotes cell division in root meristems. Catalyzes the biosynthesis of ceramide sphingolipids with C(16) to C(28) fatty acids, structural membrane lipids involved in membrane trafficking (e.g. early endosomes) and cell polarity (e.g. polar auxin transport related peoteins); mostly active with t18:0 and saturated very long saturated fatty acids (C24:0 and C26:0), such as long-chain base (LCB) phytosphingosine (t18:0), lignoceroyl- and hexacosanoyl-CoAs. Mediates resistance to sphinganine-analog mycotoxins (SAMs, e.g. fumonisin B(1)) by restoring the sphingolipid biosynthesis. Could salvage the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi apparatus in ceramides-depleted cells after SAM exposure. May prevent precocious cell death by delaying PR1 accumulation during aging. Contributes to hypoxic conditions tolerance (e.g. submergences), especially in the dark, by promoting the formation of very-long-chain (VLC) ceramide species (22:1, 24:1 and 26:1) and of VLC unsaturated ceramides, which are modulating CTR1-mediated ethylene signaling leading to endoplasmic reticulum (ER)-to-nucleus translocation of EIN2 and EIN3.

Gene References into Functions

Findings indicate that AtORM1 and AtORM2 mediation of serine palmitoyltransferase (SPT) activity differentially regulates functionally distinct ceramide synthase activities as part of a broader sphingolipid homeostatic regulatory network. PMID: 27506241
The role of At3g25540 in sphingolipid metabolism, mycotoxin resistance and cell death. PMID: 26276842
Spontaneous cell death in the loh1 line is triggered either by the accumulation of free trihydroxy sphingoid bases or ceramide species with C(16) fatty acid.[LOH1] PMID: 21883234

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity

Expressed ubiquitously at high levels. Not observed in pollen.

Database Links

KEGG: ath:AT3G25540

STRING: 3702.AT3G25540.1

UniGene: At.6098

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