LOH2 Antibody

Code CSB-PA873282XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LOH2 Polyclonal antibody
Uniprot No.
Target Names
LOH2
Alternative Names
LOH2; LAG1; LAG2; At3g19260; MVI11.18; Ceramide synthase LOH2; CS2; CSI; Protein LONGEVITY ASSURANCE GENE ONE HOMOLOG 2; LAG One Homolog 2; LAG1 homolog 2; LAG1 longevity assurance homolog 2
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LOH2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Prevents cell division in root meristems and promotes salicylic acid (SA) production and hypersensitive response (HR). Catalyzes the biosynthesis of ceramide sphingolipids with C(16) fatty acids, structural membrane lipids involved in membrane trafficking (e.g. early endosomes) and cell polarity (e.g. polar auxin transport related peoteins); accepts only C16:0 fatty acids, but with a wide range of d18 sphingoid bases, such as sphinganine (d18:0) and palmitoyl-CoA. Mediates resistance to sphinganine-analog mycotoxins (SAMs, e.g. fumonisin B(1)) by restoring the sphingolipid biosynthesis. Could salvage the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi apparatus in ceramides-depleted cells after SAM exposure. Contributes to hypoxic conditions tolerance (e.g. submergences), especially in the dark, by promoting the formation of very-long-chain (VLC) ceramide species (22:1, 24:1 and 26:1) and of VLC unsaturated ceramides, which are modulating CTR1-mediated ethylene signaling leading to endoplasmic reticulum (ER)-to-nucleus translocation of EIN2 and EIN3.
Gene References into Functions
  1. The role of At3g19260 in sphingolipid metabolism, mycotoxin resistance and cell death. PMID: 26276842
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity
Expressed ubiquitously with highest levels in pollen.
Database Links

KEGG: ath:AT3G19260

STRING: 3702.AT3G19260.1

UniGene: At.14736

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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