LEC2 Antibody

Code CSB-PA631971XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LEC2 Polyclonal antibody
Uniprot No.
Target Names
LEC2
Alternative Names
LEC2 antibody; At1g28300 antibody; F3H9.5B3 domain-containing transcription factor LEC2 antibody; Protein LEAFY COTYLEDON 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LEC2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription regulator that plays a central role in embryo development. Required for the maintenance of suspensor morphology, specification of cotyledon identity, progression through the maturation phase and suppression of premature germination. Ectopic expression is sufficient to promote somatic embryogenesis.
Gene References into Functions
  1. senescence-inducible and triggers the key metabolic steps that increase triacylglycerol accumulation in leaves PMID: 25790072
  2. LEC2 overexpression modifies the morphology and anatomy of leaves by promoting differentiation toward an embryonic pathway. PMID: 23780897
  3. LEC2 alters: (1) the delivery of photosynthates from the seed coat to the embryo, (2) carbon partitioning towards different storage compounds, (3) the rate of starch synthesis, and (4) degradation in developing seeds and germination capacity of dry seeds. PMID: 21665323
  4. Transcriptional regulation of Arabidopsis LEAFY COTYLEDON2 involves a cis-element that regulates trimethylation of histone H3 at lysine-27. PMID: 22080598
  5. Role and expression pattern of LEC2 in seed development PMID: 22228409
  6. revealed differential expression of LEC2 transcripts within a 30 days time course of somatic embryo development. PMID: 19763577
  7. Loss of embryogenic potential in the lec2 mutant in vitro is not related to the distribution of exogenously applied auxin. PMID: 16034595
  8. Data show that the induction of LEAFY COTYLEDON 2 leads to the accumulation of storage oil and seed specific mRNAs in leaves, and increases the expression of the seed regulators namely, LEC1, FUS3, and ABI3. PMID: 16107256
  9. demonstrate that LEC2 directly controls a transcriptional program involved in the maturation phase of seed development PMID: 16492731
  10. WRINKLED 1 is a target of LEAFY COTYLEDON2 and is necessary for the regulatory action on fatty acid metabolism. [Leafy Cotyledon2] [LEC2] PMID: 17419836
  11. LEC2 causes rapid changes in auxin responses and induces cellular differentiation characteristic of the maturation phase. PMID: 18287041

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Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT1G28300

STRING: 3702.AT1G28300.1

UniGene: At.26290

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