let-60 Antibody

Code CSB-PA340505XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans let-60 Polyclonal antibody
Uniprot No.
Target Names
let-60
Alternative Names
let-60 antibody; lin-34 antibody; ZK792.6Ras protein let-60 antibody; Abnormal cell lineage protein 34 antibody; Lethal protein 60 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans let-60 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The level of let-60 controls the switch between vulval and hypodermal cell fates during C.elegans vulval induction. May stimulate the guanine nucleotide exchange factor (GEF) activity of rap-1. May induce nuclear condensation.
Gene References into Functions
  1. Expression of activated LET-60/Ras in the neighboring duct cell, but not in the G1 or G2 cells, is sufficient to rescue sos-1 delamination defects, revealing that Ras acts non-cell-autonomously to permit G1 delamination. PMID: 25371363
  2. Canal cell asymmetry and let-60/Ras signaling influence which of two equivalent precursors will attach to the canal cell. PMID: 21771815
  3. LET-60 (Ras) is identified as a critical RGEF-1b substrate-effector in sensory neurons. PMID: 21482356
  4. LET-60 Ras GTPase might act with FLI-1 to control germ line morphogenesis PMID: 18485202
  5. Goa-1 Goalpha and let-60 Ras act in parallel to gpc-1 in olfactory pathway. PMID: 19189947

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Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
Small GTPase superfamily, Ras family
Tissue Specificity
Expressed in body wall muscles and in the nervous system including ganglion, nerve ring dorsal and ventral nerve cords, motor neurons and sensory tail neurons.
Database Links

KEGG: cel:CELE_ZK792.6

STRING: 6239.ZK792.6

UniGene: Cel.19758

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