Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IMDH1 Polyclonal antibody

Uniprot No.

Q9FMT1

Target Names

IMDH1

Alternative Names

IMDH1 antibody; IMD1 antibody; IPMDH1 antibody; MAM-D1 antibody; At5g14200 antibody; MUA22.203-isopropylmalate dehydrogenase 1 antibody; chloroplastic antibody; 3-IPM-DH 1 antibody; AtIMDH1 antibody; IMDH 1 antibody; EC 1.1.1.85 antibody; Beta-IPM dehydrogenase 1 antibody; Isopropylmalate dehydrogenase 1 antibody; AtIMD1 antibody; Methylthioalkylmalate dehydrogenase 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IMDH1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in both glucosinolate and leucine biosynthesis; catalyzes the oxidative decarboxylation step in both leucine biosynthesis (primary metabolism) and methionine chain elongation of glucosinolates (specialized metabolism). Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate, 3-IPM) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Required during pollen development and involved in embryo sac development. More active on 3-isopropylmalate and NAD(+) than towards D-malate.

Gene References into Functions

AtLeuC1 and AtIMD1 encode methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase, respectively. These enzymes have roles in controlling metabolism from methionine to primary and methionine-related secondary metabolites. [AtIMD1] PMID: 19493961
Disruption of AtIPMDH1 in Arabidopsis caused a dramatic decrease in the concentrations of glucosinolates with side chains of four or more carbons, and a decrease in free leucine levels. PMID: 19674406
IPMDH is involved in leucine biosynthetic pathway. Three IPMDH ORF sequences designated as AtIMD1, AtIMD2, and AtIMD3 are localized in plastids. AtIMD1 transcripts were the highest in roots. [AtIMD1; IPMDH] PMID: 15849421

Subcellular Location

Plastid, chloroplast stroma.

Protein Families

Isocitrate and isopropylmalate dehydrogenases family

Tissue Specificity

Highly expressed in seedlings, leaves, stems and roots and, to a lower extent, in flowers, pollen and siliques.

Database Links

KEGG: ath:AT5G14200

STRING: 3702.AT5G14200.1

UniGene: At.23937

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IMDH1 Antibody

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