IMDH1 Antibody

Datasheet

Code	CSB-PA887816XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IMDH1 Polyclonal antibody

Uniprot No.

Q9FMT1

Target Names

IMDH1

Alternative Names

IMDH1 antibody; IMD1 antibody; IPMDH1 antibody; MAM-D1 antibody; At5g14200 antibody; MUA22.203-isopropylmalate dehydrogenase 1 antibody; chloroplastic antibody; 3-IPM-DH 1 antibody; AtIMDH1 antibody; IMDH 1 antibody; EC 1.1.1.85 antibody; Beta-IPM dehydrogenase 1 antibody; Isopropylmalate dehydrogenase 1 antibody; AtIMD1 antibody; Methylthioalkylmalate dehydrogenase 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IMDH1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in both glucosinolate and leucine biosynthesis; catalyzes the oxidative decarboxylation step in both leucine biosynthesis (primary metabolism) and methionine chain elongation of glucosinolates (specialized metabolism). Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate, 3-IPM) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Required during pollen development and involved in embryo sac development. More active on 3-isopropylmalate and NAD(+) than towards D-malate.

Gene References into Functions

AtLeuC1 and AtIMD1 encode methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase, respectively. These enzymes have roles in controlling metabolism from methionine to primary and methionine-related secondary metabolites. [AtIMD1] PMID: 19493961
Disruption of AtIPMDH1 in Arabidopsis caused a dramatic decrease in the concentrations of glucosinolates with side chains of four or more carbons, and a decrease in free leucine levels. PMID: 19674406
IPMDH is involved in leucine biosynthetic pathway. Three IPMDH ORF sequences designated as AtIMD1, AtIMD2, and AtIMD3 are localized in plastids. AtIMD1 transcripts were the highest in roots. [AtIMD1; IPMDH] PMID: 15849421

Subcellular Location

Plastid, chloroplast stroma.

Protein Families

Isocitrate and isopropylmalate dehydrogenases family

Tissue Specificity

Highly expressed in seedlings, leaves, stems and roots and, to a lower extent, in flowers, pollen and siliques.

Database Links

KEGG: ath:AT5G14200

STRING: 3702.AT5G14200.1

UniGene: At.23937

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Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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IMDH1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background