lexA Antibody

Code CSB-PA333578XA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) lexA Polyclonal antibody
Uniprot No.
Target Names
lexA
Alternative Names
lexA antibody; dinR antibody; BSU17850LexA repressor antibody; EC 3.4.21.88 antibody; SOS regulatory protein DinR antibody
Raised in
Rabbit
Species Reactivity
Bacillus subtilis (strain 168)
Immunogen
Recombinant Bacillus subtilis (strain 168) lexA protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Represses dinA, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'; some genes have a tandem consensus sequence and their binding is cooperative. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair; autocleavage is maximal at pH 11 in the absence of RecA and ssDNA.
Gene References into Functions
  1. B.subtilis SOS operator sequence requirements for binding the Bacillus subtilis LexA protein. PMID: 16269821
Protein Families
Peptidase S24 family
Database Links
CUSABIO guaranteed quality
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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