LFY Antibody

Code CSB-PA616777XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LFY Polyclonal antibody
Uniprot No.
Target Names
LFY
Alternative Names
LFY antibody; At5g61850 antibody; MAC9.18 antibody; Protein LEAFY antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LFY protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1. Is required subsequently for the transition of an inflorescence meristem into a floral meristem, by an immediate upstream regulation of the ABC classes of floral homeotic genes. Activates directly APETALA1, CAULIFLOWER and AGAMOUS, and indirectly APETALA3 and PISTILLATA with the cooperation of UFO.
Gene References into Functions
  1. LFY and AP1 are conserved floral regulators that act nonredundantly in C. hirsuta, such that LFY has more obvious roles in floral and leaf development in C. hirsuta than in A. thaliana. PMID: 28098947
  2. LFY and AP1/CAL act as part of an incoherent feed-forward loop, a network motif where two interconnected pathways or transcription factors act in opposite directions on a target gene, to control the establishment of a stable developmental program for the formation of flowers. PMID: 28385730
  3. WRKY71 activity hastens flowering via the direct activation of FT and LFY. PMID: 26643131
  4. A SAM oligomerization domain shapes the genomic binding landscape of the LEAFY transcription factor. PMID: 27097556
  5. Data suggest that helix-turn-helix transcription factor LEAFY (LFY) and the MADS box transcription factor APETALA1 (AP1)together orchestrate the switch to flower formation and morphogenesis by altering transcriptional programs. PMID: 26096587
  6. LEAFY controls auxin response pathways in floral primordium formation. PMID: 23572147
  7. must act in complexes that contain at least two LFY molecules; the N-terminus is essential for stabilization PMID: 22507399
  8. Studies indicate that LEAFY is a master regulator of flowering. PMID: 22451042
  9. LFY activity affects adaxial pedicel identity and cell elongation. LFY mutation suppresses the crm1 pedicel length and orientation phenotypes. PMID: 22050454
  10. The SWI2/SNF2 ATPases physically interact with two direct transcriptional activators of class B and class C gene expression, LEAFY (LFY) and SEPALLATA3 (SEP3). PMID: 22323601
  11. LEAFY binding site is essential for proper photoperiodic activation of APETALA1 PMID: 21623976
  12. A biophysical model describing LFY DNA binding specificity in vitro was built that accurately predicts in vivo LFY binding sites in the Arabidopsis thaliana genome. PMID: 21515819
  13. Data show that the direct LEAFY target LATE MERISTEM IDENTITY2 (LMI2) has a role in the meristem identity transition, and acts together with LEAFY to activate APETALA1. PMID: 21750030
  14. The motif analyses reveal a possible mechanism for stage-specific LFY recruitment and suggest a role for LFY in overcoming polycomb repression. PMID: 21497757
  15. FT regulates SOC1 expression, and SOC1 regulates LFY expression PMID: 15695467
  16. The transcription factor LFY controls the switch from vegetative to reproductive development. PMID: 16554366
  17. Mustard plsnt LcrLFY has diverged from A. thaliana in both the cis-regulatory and protein-coding regions which contribute to the evolution of rossette flowering. PMID: 16915521
  18. GA(4) is the active GA in the regulation of LFY transcription and Arabidopsis flowering time under short-day conditions. PMID: 16920780
  19. Activation of the LFY transcriptrion factor is required. PMID: 18287201
  20. Results report crystal structures for the DNA-binding domain of Arabidopsis thaliana LEAFY bound to two target promoter elements. PMID: 18784751
  21. LEAFY activates the expression of floral organ identity genes APETALA3, AGAMOUS and APETALA1 PMID: 9783581

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Subcellular Location
Nucleus.
Protein Families
FLO/LFY family
Tissue Specificity
Expressed uniformly throughout the young floral primordia.
Database Links

KEGG: ath:AT5G61850

STRING: 3702.AT5G61850.1

UniGene: At.55671

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