lgg-2 Antibody

Code CSB-PA630489XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans lgg-2 Polyclonal antibody
Uniprot No.
Target Names
lgg-2
Alternative Names
lgg-2 antibody; ZK593.6Protein lgg-2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans lgg-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin-like modifier involved in the formation of autophagosomal vacuoles (autophagosomes). When lipidated mediates tethering between adjacent membranes and stimulates membrane fusion. Less effective at promoting membrane fusion than lgg-1. Acts upstream of the autophagy protein epg-5 in the aggrephagy pathway, which is the macroautophagic degradation of ubiquitinated protein aggregates, and preferentially interacts with autophagy proteins and substrates containing LIR motifs to mediate autophagosome formation and protein aggregate degradation. In particular binds to components of an atg-5-lgg-3-atg-16 complex to regulate autophagosome formation and cargo sequestration. Required for the degradation of specific sqst-1-containing aggregates during embryogenesis and the early stages of larval development. Involved in allophagy, which is an autophagic process in which paternal mitochondria and organelles are degraded during fertilization, and moreover is required for the degradation of lgg-1-positive allophagic autophagosomes in embryos. Involved in xenophagy, the autophagy-mediated degradation of pathogens and pathogen products, such as toxins. Also plays a role in membrane-pore repair. Through HOPS complex subunit vps-39, tethers lysosomes with autophagosomes to form autolysosomes. Plays a role in the distribution and clearance of germ cell specific P-granules from somatic cells to ensure exclusive localization of the P-granules in germ cells. Essential for dauer development and life-span extension.
Gene References into Functions
  1. the major contribution of LGG-2 in sperm-inherited organelle clearance resides in its capacity to mediate the retrograde transport of autophagosomes rather than their fusion with acidic compartments. PMID: 25922527
  2. LGG-2 interacts with VPS-39 to tether autophagosomes and lysosomes. PMID: 24374177
Subcellular Location
Cytoplasmic vesicle, autophagosome. Cytoplasm. Cell membrane; Lipid-anchor.
Protein Families
ATG8 family
Database Links

KEGG: cel:CELE_ZK593.6

STRING: 6239.ZK593.6a

UniGene: Cel.18191

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