LIG6 Antibody

Code CSB-PA870209XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LIG6 Polyclonal antibody
Uniprot No.
Target Names
LIG6
Alternative Names
LIG6 antibody; At1g66730 antibody; F4N21.14 antibody; DNA ligase 6 antibody; AtLIG6 antibody; DNA ligase VI antibody; EC 6.5.1.1 antibody; Ligase 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LIG6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (Probable). Required to maintain seed viability (e.g. longevity and storability) and during seed germination, probably by repairing DNA damage accumulated during seed development, storage and/or imbibition. Facilitates seed germination in cold conditions (2 degrees Celsius) and under oxidative stress (e.g. menadione, a genotoxic agent). Involved in repair of X-ray-induced damage.; Limits stable root transformation by A.tumefaciens T-DNA.
Gene References into Functions
  1. Studies reveal that AtLIG6 (AT1G66730) is not only required for rapid seed germination under optimal conditions but also becomes an important determinant of germination performance and seed quality PMID: 20584150
Subcellular Location
Nucleus.
Protein Families
ATP-dependent DNA ligase family
Tissue Specificity
Mostly expressed in buds and flowers, and, to a lower extent, in stems, leaves, siliques and seeds.
Database Links

KEGG: ath:AT1G66730

STRING: 3702.AT1G66730.1

UniGene: At.35739

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