ligD Antibody

Datasheet

Code	CSB-PA620474XA01MVX
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) ligD Polyclonal antibody

Uniprot No.

A0R3R7

Target Names

ligD

Alternative Names

ligD antibody; MSMEG_5570 antibody; MSMEI_5419Multifunctional non-homologous end joining protein LigD antibody; NHEJ DNA polymerase) [Includes: DNA repair polymerase antibody; Pol antibody; Polymerase/primase); 3'-phosphoesterase antibody; 3'-ribonuclease/3'-phosphatase antibody; PE); DNA ligase antibody; Lig antibody; EC 6.5.1.1 antibody; Polydeoxyribonucleotide synthase [ATP])] antibody

Raised in

Rabbit

Species Reactivity

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Immunogen

Recombinant Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) ligD protein

Immunogen Species

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

With Ku forms a non-homologous end joining (NHEJ) repair enzyme which repairs blunt-end and 5'-overhang DNA double strand breaks (DSB) with about 50% fidelity, and DSB with non-complementary 3' ends. Plays a partial role in NHEJ during 3'-overhang repair. NHEJ repairs DSB with blunt ends and 5' overhangs with a high level of nucleotide insertion/deletion, without a need for microhomology. Acts as a DNA ligase on singly nicked dsDNA, as a DNA-directed DNA polymerase on 5' overhangs, and adds non-templated nucleotides to 3' overhangs (terminal transferase). Fills in gaps in dsDNA, prefers a 5'-phosphate in the gap. Site-directed mutations leading to ligase loss alter the bias from insertion to deletion mutations, and indicate another ligase (LigC1 and/or LigC2) can compensate.; The preference of the polymerase domain for rNTPs over dNTPs may be advantageous in dormant cells, where the dNTP pool may be limiting.; The ligase activity is required for replication of viruses with short cos ends (4 bases) such as Mycobacterium phage Omega and Corndog, but not D29 which has a 9 base cos end. Stimulates dsDNA end joining by LigD; when expressed with endogenous or Mycobacterium phage Omega Ku, can reconstitute NHEJ in S.cerevisiae.

Protein Families

LigD polymerase family; LigD 3'-phosphoesterase family; ATP-dependent DNA ligase family

Database Links

KEGG: msg:MSMEI_5419

STRING: 246196.MSMEG_5570

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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