lin-53 Antibody

Code CSB-PA309714XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans lin-53 Polyclonal antibody
Uniprot No.
Target Names
lin-53
Alternative Names
lin-53 antibody; rba-2 antibody; K07A1.12 antibody; Probable histone-binding protein lin-53 antibody; Abnormal cell lineage protein 53 antibody; Synthetic multivulva protein p48 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans lin-53 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Required for hcp-3 and his-1 stabilization, localization of hcp-3 to centromeres and for proper chromosome segregation. Synthetic multivulva class B (synMuvB) protein. SynMuvB proteins are required to repress the induction of vulval development by Ras signaling and probably act by forming the multiprotein DRM complex that represses transcription.
Gene References into Functions
  1. RbAp46/48LIN-53 localizes to the centromere during metaphase in a CENP-AHCP-3- and M18BP1KNL-2-dependent manner, suggesting CENP-AHCP-3 loading may occur before anaphase. PMID: 26904949
  2. through ectopic expression of single transcription factors (che-1) study found the identity of mitotic germ cells can be directly converted into that of specific neuron types; this reprogramming requires removal of LIN-53 PMID: 21148348
Subcellular Location
Nucleus. Chromosome, centromere.
Protein Families
WD repeat RBAP46/RBAP48/MSI1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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