lmn-1 Antibody

Code CSB-PA637421XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans lmn-1 Polyclonal antibody
Uniprot No.
Target Names
lmn-1
Alternative Names
lmn-1 antibody; lam-1 antibody; DY3.2 antibody; Lamin-1 antibody; Ce-lamin antibody; CeLam-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans lmn-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Major component of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Provides a framework for the nuclear envelope and probably also interacts with chromatin. Essential to maintain the shape and integrity of the nucleus, and for DNA replication. Involved in spatial organization of nuclear pore complexes. It is not a target for ced-3 during apoptosis, suggesting that lamin cleavage is not essential for apoptosis in C.elegans.
Gene References into Functions
  1. The Caenorhabditis elegans lamin, LMN-1, is required for nuclear migration and interacts with the nucleoplasmic domain of the SUN protein UNC-84. PMID: 25057012
  2. In LMN-1 Y59C (a point mutation)-expressing worms, there is abnormal retention at the nuclear envelope of a gene array bearing a muscle-specific promoter. This correlates with altered expression of a number of muscle-specific genes. PMID: 21962710
  3. A laminopathic mutation disrupting lamin filament assembly causes disease-like phenotypes in Caenorhabditis elegans. PMID: 21653823
  4. documented that the single nuclear lamin of C. elegans assembles in low ionic strength Tris-HCl buffer into stable 10-nm filaments that exhibit a normal intermediate filament morphology PMID: 12488092
  5. Both lamin Dm0 and C. elegans Ce-lamin bind directly to histone H2A in vitro and this binding requires a nuclear localization signal PMID: 17148572

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Subcellular Location
Nucleus envelope. Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Protein Families
Intermediate filament family
Tissue Specificity
Ubiquitous. Expressed in all cells, except in cells undergoing spermatogenesis.
Database Links

KEGG: cel:CELE_DY3.2

STRING: 6239.DY3.2.2

UniGene: Cel.17828

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