lnt Antibody

Code CSB-PA338556XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) lnt Polyclonal antibody
Uniprot No.
Target Names
lnt
Alternative Names
lnt; cutE; b0657; JW0654; Apolipoprotein N-acyltransferase; ALP N-acyltransferase; Copper homeostasis protein CutE
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) lnt protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation. Utilizes a two-step reaction via a ping-pong mechanism. Lnt undergoes covalent modification in the presence of phospholipids, resulting in a thioester acyl-enzyme intermediate. It then transfers the acyl chain to the amine group of the N-terminal diacylglyceryl-modified cysteine of apolipoprotein, leading to the formation of mature triacylated lipoprotein. In vitro, can utilize the phospholipids phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidic acid (PA) or cardiolipin (CL). PE is the most efficient acyl donor.
Gene References into Functions
  1. High-resolution crystal structures were obtained for membrane integral Lnt. Thermal stability was measured by circular dichroism. The detergent-solubilized form possessed N-acylation activity. PMID: 29573991
  2. data suggest that amino acids located on flexible cytoplasmic and periplasmic loops, predicted to be membrane embedded, are required for efficient N-acylation of lipoproteins PMID: 25471278
  3. Lnt is an essential protein in E. coli and the lethality is partially because of the retention of apoLpp in the plasma membrane PMID: 15513925
  4. Essential residues in Lnt were identified by using site-directed mutagenesis and complementation assay; besides the catalytic triad E267-K335-C387, 4 residues that directly affect the modification of Braun's lipoprotein Lpp are required for Lnt function. PMID: 17416655
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
CN hydrolase family, Apolipoprotein N-acyltransferase subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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