LOT6 Antibody

Code CSB-PA156292XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LOT6 Polyclonal antibody
Uniprot No.
Target Names
LOT6
Alternative Names
LOT6 antibody; YLR011W antibody; NAD(P)H-dependent FMN reductase LOT6 antibody; FMN reductase LOT6 antibody; EC 1.5.1.39 antibody; Azoreductase LOT6 antibody; FMN reductase [NAD(P)H] antibody; Low temperature response protein 6 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LOT6 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has several reductase activities that are NAD(P)H-dependent and involve FMN as a cofactor, ferricyanide being the best substrate for reduction. May be involved in ferric iron assimilation.
Gene References into Functions
  1. Using hexahistidine tagged, recombinant Lot6p, the authors determined the steady-state enzyme kinetic parameters with both NADH and NADPH as electron donors; no cooperativity was observed with these substrates. PMID: 24866129
  2. LOT6 is a FMN-dependent reductase with a potential role in quinone detoxification. PMID: 17298444
  3. Lot6-proteasome complex can be regarded as a redox switch in which the quinone reductase acts as a sensor for oxidative stress PMID: 19029946
  4. Hydride transfer, and not an enzyme conformational change, is rate-determining in the Lot6 reductive half-reaction. In the oxidative half-reaction, reduced enzyme reacted in a single phase with six quinone substrates tested. PMID: 19618916
  5. The authors propose that Lot6p is directly involved in the control of the apoptosis-like cell death in yeast. PMID: 19709309

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Subcellular Location
Cytoplasm. Nucleus.
Database Links

KEGG: sce:YLR011W

STRING: 4932.YLR011W

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