LOX1 Antibody

Code CSB-PA130151XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LOX1 Polyclonal antibody
Uniprot No.
Target Names
LOX1
Alternative Names
LOX1 antibody; At1g55020 antibody; F14C21.3 antibody; F14C21.54 antibody; T24C10.13Linoleate 9S-lipoxygenase 1 antibody; EC 1.13.11.58 antibody; Lipoxygenase 1 antibody; AtLOX1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LOX1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
9S-lipoxygenase that can use linoleic acid or linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Function as regulators of root development by controlling the emergence of lateral roots. 9S-lypoxygenase-derived oxylipins may play an antagonistic role to ethylene signaling in the control of responses involving oxidative stress, lipid peroxidation and plant defense. LOX1-derived oxylipins may be involved in stress signaling from roots to shoots in response to cadmium exposure. 9S-lypoxygenase-derived oxylipins are engaged during infection to control the balance between salicylic acid (SA) and jasmonate (JA) signaling to facilitate infection by the fungal pathogen Fusarium graminearum. 9S-lypoxygenase-derived oxylipins activate brassinosteroid signaling to promote cell wall-based defense and limit pathogen infection. The LOX1-derived compound (9S)-hydroperoxy-(10E,12Z,15Z)-octadecatrienoate protects plant tissues against infection by the bacterial pathogen Pseudomonas syringae pv tomato DC3000. The LOX1-derived oxylipins are required to trigger stomatal closure in response to both infection by the bacterial pathogen Pseudomonas syringae pv tomato DC3000, and the pathogen-associated molecular pattern (PAMP) flagellin peptide flg22. Contributes to the oxidation of free fatty acids during seed aging.
Gene References into Functions
  1. LOX1 and alpha-DOX1 contribute to plant defense by modulating hormonal responses. [LOX1] PMID: 22199234
Subcellular Location
Cytoplasm.
Protein Families
Lipoxygenase family
Tissue Specificity
Seedlings, roots, leaves, and flowers (at protein level). Expressed in guard cells.
Database Links

KEGG: ath:AT1G55020

STRING: 3702.AT1G55020.1

UniGene: At.19984

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