Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LECRK19 Polyclonal antibody

Uniprot No.

Q9LSR8

Target Names

LECRK19

Alternative Names

LECRK19 antibody; DORN1 antibody; P2K1 antibody; At5g60300 antibody; F15L12.17 antibody; L-type lectin-domain containing receptor kinase I.9 antibody; LecRK-I.9 antibody; LecRK79 antibody; EC 2.7.11.1 antibody; Protein DOES NOT RESPOND TO NUCLEOTIDES 1 antibody; Purinoreceptor kinase 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LECRK19 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential receptor for extracellular ATP. Binds ATP with high affinity through its extracellular legume-lectin like region. Is required for ATP-induced intracellular calcium response, mitogen-activated protein kinase 3 (MPK3) and MPK6 activation and ATP-induced gene expression. May play a variety of roles in stress resistance. May be involved in protein-protein interactions with RGD motif-containing proteins as potential ligands. Plays probably a structural and signaling role at the plant cell surfaces.; Involved in resistance response to the pathogenic oomycetes Phytophthora infestans and Phytophthora capsici.

Gene References into Functions

Mutation of DORN1 phosphorylation sites on RBOHD eliminates the ability of ATP to induce stomatal closure. The data implicate purinergic signaling via DORN1 in the control of stomatal aperture. PMID: 29273780
The role of LecRK-I.9 is discussed with regard to the potential molecular mechanisms linking jasmonic acid signalling to cell wall damage and/or extracellular ATP perception. PMID: 27399963
X-ray crystallographic studies of the extracellular domain of the first plant ATP receptor, DORN1, and the orthologous protein from Camelina sativa, have been presented. PMID: 27710944
the genetic and biochemical evidence for the role of DORN1 in extracellular ATP signalling, placing this within the wider context of extracellular ATP signalling during plant stress responses. [Review] PMID: 25301072
LecRK-I.9 insertion mutants gain susceptibility to Phytophthora. Overexpression enhances resistance. Observations strongly suggest that LecRK-I.9 plays a role in disease resistance by stabilizing the cell wall-plasma membrane continuum. [LecRK-I.9] PMID: 21483488
DORN1 is essential for perception of extracellular ATP and likely plays a variety of roles in plant stress resistance. PMID: 24436418
The lectin receptor kinase encoded by At5g60300 is involved in protein-protein interactions with RGD-containing proteins as potential ligands, and may play a structural and signaling role at the plant cell surfaces. PMID: 16361528

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family; Leguminous lectin family

Tissue Specificity

Expressed in leaf petioles.

Database Links

KEGG: ath:AT5G60300

STRING: 3702.AT5G60300.3

UniGene: At.21114

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LECRK19 Antibody

Product Details

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Target Background