Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LSD1 Polyclonal antibody

Uniprot No.

P94077

Target Names

LSD1

Alternative Names

LSD1 antibody; CHS4 antibody; At4g20380 antibody; F9F13.30 antibody; Protein LSD1 antibody; Protein CHILLING SENSITIVE 4 antibody; Protein LESION SIMULATING DISEASE 1 antibody; AtLSD1 antibody; Putative zinc finger LSD1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LSD1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Negative regulator of reactive oxygen-induced cell death, cold stress-induced cell death, pathogen-induced hypersensitive response (HR), basal disease resistance. May be involved in the induction of the copper/zinc superoxide dismutase CSD1 and CSD2 that detoxify accumulating superoxide before the reactive oxygen species (ROS) can trigger a cell death cascade. LSD1 and LOL1 have antagonistic effects on CSD1 and CSD2 accumulation to regulate oxidative stress-induced cell death. Antagonizes the function of BZIP10, a positive regulator of cell death, by interacting in the cytoplasm and preventing its nuclear localization. Controls lysigenous aerenchyma in hypocotyls under root hypoxia. Required for leaf acclimation in response to excess excitation energy.

Gene References into Functions

LSD1 plays a dual role within the cell by acting as a condition-dependent scaffold protein and as a transcription regulator. PMID: 28555890
lsd1-catalase interaction plays an important role in regulating programmed cell death (PCD) in Arabidopsis. PMID: 23958864
These findings reveal a role of LSD1 in regulating cell death trigged by cold stress and a link between cold stress responses and ROS-associated signaling. PMID: 20456049
the roles of LSD1 in light acclimation and in restricting pathogen-induced cell death are functionally linked PMID: 15347794
Use in the implementation of bimolecular fluorescence complementation for visualization of protein-protein interactions in plant cells. PMID: 15469500
LSD1 likely functions as a cellular hub, where its interaction with AtbZIP10 and additional, as yet unidentified, proteins contributes significantly to plant oxidative stress responses PMID: 16957775
The balanced activities of LSD1, EDS1, and PAD4 regulate lysigenous aerenchyma formation in response to hypoxia. PMID: 18055613

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Subcellular Location

Cytoplasm. Nucleus.

Tissue Specificity

Expressed in cotyledons, roots, rosette leaves, stems, inflorescences and flowers.

Database Links

KEGG: ath:AT4G20380

UniGene: At.24187

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LSD1 Antibody

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