Su(var)3-3 Antibody

Code CSB-PA894284XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Su(var)3-3 Polyclonal antibody
Uniprot No.
Target Names
Su(var)3-3
Alternative Names
Su(var)3-3 antibody; Hdm antibody; LSD1 antibody; CG17149 antibody; Possible lysine-specific histone demethylase 1 antibody; EC 1.-.-.- antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Su(var)3-3 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for heterochromatic gene silencing. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylated 'Lys-4' of histone H3. May also demethylate 'Lys-9' of histone H3, Plays a role in the repression of neuronal genes.
Gene References into Functions
  1. identified lysine-specific demethylase 1 (lsd1) and CoRest as differentiation regulators PMID: 25512561
  2. Lsd1 functions to repress decapentaplegic (dpp) expression in adult germaria PMID: 21482791
  3. found that the Lysine Specific Demethylase 1 gene (lsd1) was important for the proper cell type-specific development of wing patterning PMID: 21146519
  4. Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development. PMID: 17462898
Subcellular Location
Nucleus. Chromosome. Note=In early cleavage, protein displays a uniform nuclear distribution. At metaphase, protein is detected at the chromosome periphery, and between the chromosomes during anaphase. A rather uniform interphase chromatin association is detected until syncytial blastoderm. When cellular blastoderm is formed (cycle 14), protein is preferably associated with pericentric heterochromatin situated at the apical pole of blastoderm nuclei. Later in development, protein is only found over euchromatin. In the germline precursor pole cells, protein remains uniformly distributed within the nucleus.
Protein Families
Flavin monoamine oxidase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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