RKR1 Antibody

Code CSB-PA179753XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RKR1 Polyclonal antibody
Uniprot No.
Target Names
RKR1
Alternative Names
RKR1 antibody; LTN1 antibody; YMR247C antibody; YM9408.09C antibody; YM9920.01CE3 ubiquitin-protein ligase listerin antibody; EC 2.3.2.27 antibody; RING domain mutant killed by rtf1 deletion protein 1 antibody; RING-type E3 ubiquitin transferase listerin antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RKR1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non-stop proteins) and other translation arrest products induced by poly-lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.
Gene References into Functions
  1. findings can explain the proteotoxicity of ribosome-stalled polypeptides and demonstrate the essential role of the RQC in maintaining proteostasis PMID: 26934223
  2. Our results indicate that Rkr1 plays the primary role in targeting the tested model ER-targeted nonstop and translationally stalled proteins for degradation PMID: 26055716
  3. Ltn1 and Ubp3-Bre5 likely contribute to adapt ribophagy activity to both nutrient supply and protein translation. PMID: 24616224
  4. Data show that Ltn1 E3 ligase has an elongated form and presents a continuum of conformational states about two flexible hinge regions. PMID: 23319619
  5. These results identify a new participant (Rkr1) in a protein ubiquitylation pathway within the nucleus that acts to modulate chromatin function and transcription. PMID: 17283062

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Subcellular Location
Nucleus. Cytoplasm, cytosol.
Protein Families
LTN1 family
Database Links

KEGG: sce:YMR247C

STRING: 4932.YMR247C

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