LUH Antibody

Datasheet

Code	CSB-PA933418XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LUH Polyclonal antibody

Uniprot No.

O48847

Target Names

LUH

Alternative Names

LUH antibody; MUM1 antibody; At2g32700 antibody; F24L7.16Transcriptional corepressor LEUNIG_HOMOLOG antibody; Protein MUCILAGE-MODIFIED 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LUH protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Transcription repressor subunit of the SEU-SLK1 and SEU-SLK2 transcriptional corepressor of abiotic stress (e.g. salt and osmotic stress) response genes, by means of an epigenetic process involving histone modification (e.g. H3K9 and H3K14 acetylation), probably by recruiting HDAC, to facilitate the condensation of chromatin thus preventing transcription at the target genes. Can also act as a transcription activator. Implicated in embryo and floral development. Involved in post-synthesis cell wall modifications necessary for mucilage extrusion from seeds upon imbibition, probably by promoting the expression of genes required for mucilage maturation (e.g. MUM2). Regulates the maintenance on leaf polarity and meristem activity as well as the initiation of embryonic shoot apical meristem (SAM) development.

Gene References into Functions

LUH is allelic to MUM1, and MUM2 is a likely target of LUH regulation. The transcriptional corepressor LEUNIG (LUG) is functionally interchangeable with LUH, raising the possibility that LUH functions as a repressor during seed coat maturation. PMID: 21402796
that SLK1, SLK2 and LUH form a co-repressor complex. PMID: 24564815
LUH/MUM1 expression appears to be independent of other transcription factors known to regulate aspects of seed coat mucilage biology. PMID: 21518777
luh-1 mutant seeds exhibit mucilage extrusion defects, but do not exhibit significant changes in seed coat morphology or integument cell differentiation. PMID: 21362134
YABs interact with LUG and LUH. Together these proteins maintain leaf polarity and meristem activity in Arabidopsis. PMID: 19837869

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in roots, stems, leaves, seedlings, apex, flowers, siliques, flower organs and seeds (including seed coat).

Database Links

KEGG: ath:AT2G32700

STRING: 3702.AT2G32700.7

UniGene: At.21002

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Protein Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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