MKK4 Antibody

Code CSB-PA013413XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MKK4 Polyclonal antibody
Uniprot No.
Target Names
MKK4
Alternative Names
MKK4 antibody; At1g51660 antibody; F19C24.26Mitogen-activated protein kinase kinase 4 antibody; AtMKK4 antibody; MAP kinase kinase 4 antibody; EC 2.7.12.2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MKK4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the second phase of hydrogen peroxide generation during hypersensitive response-like cell death. Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. Activates by phosphorylation the downstream MPK3 and MPK6. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs. MKK4 and MKK5 participate in the regulation of floral organ abscission. Mediates osmotic-stress response via its regulation of MPK3 activity. Target of the Pseudomonas syringae type III effector HopF2.
Gene References into Functions
  1. MKK4 is a key regulator of stomatal development and patterning. PMID: 17259259
Subcellular Location
Cytoplasm. Nucleus. Plastid, chloroplast stroma.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity
Expressed higher in stems and leaves than in flowers and roots.
Database Links

KEGG: ath:AT1G51660

STRING: 3702.AT1G51660.1

UniGene: At.351

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