MAPKKK5 Antibody

Code CSB-PA826039XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MAPKKK5 Polyclonal antibody
Uniprot No.
Target Names
MAPKKK5
Alternative Names
MAPKKK5 antibody; MAP3KG antibody; At5g66850 antibody; MUD21.11Mitogen-activated protein kinase kinase kinase 5 antibody; EC 2.7.11.25 antibody; MAP3K gamma protein kinase antibody; AtMAP3Kgamma antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MAPKKK5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Mitogen-activated protein kinase (MAPK) involved in the transduction of signal between the host cell surface chitin receptor complex CERK1-LYK5 and the intracellular MAPK cascade that leads to chitin-induced immunity. Phosphorylates and activates MAPK targets (e.g. MKK4, MKK5, and possibly MKK2) when phosphorylated by PBL27 after elicitation by chitin. Required for resistance to the fungus A.brassicicola.
Subcellular Location
Cell membrane. Cytoplasm, cytosol.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
Tissue Specificity
Mostly expressed in flower buds. Also present in pollen, roots, leaves and seedlings, and, at low levels, in stems and immature siliques.
Database Links

KEGG: ath:AT5G66850

STRING: 3702.AT5G66850.1

UniGene: At.25020

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