MAP65-3 Antibody

Code CSB-PA884243XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MAP65-3 Polyclonal antibody
Uniprot No.
Target Names
MAP65-3
Alternative Names
MAP65-3 antibody; PLE antibody; At5g51600 antibody; K17N15.1565-kDa microtubule-associated protein 3 antibody; AtMAP65-3 antibody; Protein PLEIADE antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MAP65-3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Microtubule-associated protein that plays a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. Essential for the cytokinesis, especially in roots, by maintaining the integrity of the overlapped microtubules in the phragmoplast. Required during root morphogenesis. Needed for giant cell development during root knot nematode infection, where cytokinesis is initiated but not completed.
Gene References into Functions
  1. Cell cycle-regulated PLEIADE/AtMAP65-3 links membrane and microtubule dynamics during plant cytokinesis. PMID: 27420177
  2. downy mildew and powdery mildew stimulate AtMAP65-3 expression to down-regulate salicylic acid signaling for infection. PMID: 26798028
  3. MAP65-3 selectively cross-linked interdigitating microtubules (IMTs) to allow antiparallel microtubules (MTs) to be closely engaged in the phragmoplast. PMID: 21873565
  4. Data conclude that MAP65-3 contains a distinct C-terminal MT binding site with a specific role in cross-linking antiparallel MTs toward their plus ends in the phragmoplast. PMID: 22570443
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, phragmoplast. Note=Locates only to the mitotic MT arrays. Present in MT cortical arrays just before mitosis. Associates to MT in preprophase band, during anaphase, and in phragmoplast, including midzone. Distributed diffusely through the cytoplasm during metaphase only. At the end of cytokinesis, present only at the cell periphery, forming a ring around the newly formed cell plate. During root knot nematode infection, targeted to the giant cell mini cell plate.
Protein Families
MAP65/ASE1 family
Tissue Specificity
Expressed in all tissues enriched in dividing cells, such as the root and shoot apical meristem, foliar primordia, and young leaves, and embryos.
Database Links

KEGG: ath:AT5G51600

STRING: 3702.AT5G51600.1

UniGene: At.29670

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