MAD2 Antibody

Datasheet

Code	CSB-PA340327XA01SVG
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Target Background

Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MAD2 Polyclonal antibody

Uniprot No.

P40958

Target Names

MAD2

Alternative Names

MAD2 antibody; YJL030W antibody; J1256Mitotic spindle checkpoint component MAD2 antibody; Mitotic MAD2 protein antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MAD2 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Central component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Thought to be recruited to unattached kinetochores by MAD1. During checkpoint activity, MAD2 is relayed from the MAD1-MAD2 complex to the mitotic checkpoint complex (MCC). MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.

Gene References into Functions

Mad2 recruitment to the kinetochore and it's role in the spindle assembly checkpoint PMID: 27280788
the very same dimerization interface of Mad2 is required for two different, yet fundamental, consecutive steps of SAC signaling: at the kinetochores for priming Cdc20/Mad2 binding, away from kinetochores for stabilizing this complex PMID: 23000150
Mad2 is an important meiotic cell cycle regulator that ensures the timely degradation of anaphase-promoting complex substrates and the proper orchestration of the meiotic divisions PMID: 21697504
Mad2 prolongs DNA damage checkpoint arrest caused by a double-strand break via a centromere-dependent mechanism. PMID: 20096585
Data demonstrate that Mad2p localization at nuclear pore complexes is sensitive to nuclear levels of Ran and suggest that release of Mad2p from NPCs is closely linked with spindle assembly checkpoint activation in yeast. PMID: 15701789
Our biochemical and genetic evidence shows that the interaction of open and closed Mad2 is essential for the spindle assembly checkpoint and is conserved in evolution. PMID: 16818718
results surpass previously formulated objections to the Mad2-template model and predict that the release of Mad2 from Cdc20 is an energy-driven process PMID: 19143472

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Subcellular Location

Nucleus.

Protein Families

MAD2 family

Database Links

KEGG: sce:YJL030W

STRING: 4932.YJL030W

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Email: [email protected]
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Protein Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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MAD2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background