alpha-Man-IIa Antibody

Code CSB-PA632584XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) alpha-Man-IIa Polyclonal antibody
Uniprot No.
Target Names
alpha-Man-IIa
Alternative Names
alpha-Man-IIa antibody; GmII antibody; CG18802 antibody; Alpha-mannosidase 2 antibody; EC 3.2.1.114 antibody; Golgi alpha-mannosidase II antibody; AMan II antibody; Man II antibody; Golgi alpha-mannosidase IIa antibody; Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) alpha-Man-IIa protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Gene References into Functions
  1. These data show that the Drosophila metallophosphoesterase-dephosphorylated alpha-Man-II is required for the removal of the Rh1 oligosaccharide chain. PMID: 21804530
  2. Drosophila melanogaster Golgi alpha-mannosidase II mechanism seen through the structural analysis of covalent reaction intermediates PMID: 12960159
  3. high-resolution crystal structure of a nucleophile mutant of Drosophila melanogaster GMII bound to its natural oligosaccharide substrate and an oligosaccharide precursor as well as the structure of the unliganded mutant. PMID: 18599462
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyl hydrolase 38 family
Database Links

KEGG: dme:Dmel_CG18802

STRING: 7227.FBpp0081509

UniGene: Dm.2459

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