MBD5 Antibody

Code CSB-PA882917XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MBD5 Polyclonal antibody
Uniprot No.
Target Names
MBD5
Alternative Names
MBD5 antibody; At3g46580 antibody; F12A12.100Methyl-CpG-binding domain-containing protein 5 antibody; AtMBD5 antibody; MBD05 antibody; Methyl-CpG-binding protein MBD5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MBD5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional regulator that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. In addition, binds specifically methylated m(5)CpNpN but not m(5)CpNpG (N is A, T or C). Plays probably a role in gene silencing.
Gene References into Functions
  1. This study showed that AtMBD5 becomes localized to the vicinity of chromosomes with the aid of AtRAN3 during cell division. PMID: 17008347
Subcellular Location
Nucleus. Chromosome.
Tissue Specificity
Mostly expressed in flowers, and, to a lower extent, in seedlings, buds, stems and mature seeds, but barely in roots, exclusively in root meristem cells at tips (at protein level).
Database Links

KEGG: ath:AT3G46580

STRING: 3702.AT3G46580.1

UniGene: At.27178

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