MBD7 Antibody

Code CSB-PA887775XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MBD7 Polyclonal antibody
Uniprot No.
Target Names
MBD7
Alternative Names
MBD7 antibody; At5g59800 antibody; MMN10.2 antibody; Methyl-CpG-binding domain-containing protein 7 antibody; AtMBD7 antibody; MBD07 antibody; Methyl-CpG-binding protein MBD7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MBD7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional regulator that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. May directly affect chromatin structure by inducing intra- and inter- chromatin compaction via bridging over multiple methylated CpG sites. Acts as an anti-silencing factor that prevents DNA hypermethylation and gene repression. Requires high mCG density for binding. Recognizes preferentially mCGs located in transposable elements. Required for active DNA demethylation. Prefers to target genomic loci around chromocenters.
Gene References into Functions
  1. MBD7 binds to genomic regions with a high density of CG methylation.MBD7 physically associates with IDM3, and with IDM1 and IDM2 proteins.MBD7 and IDM3 prevent DNA hypermethylation and gene silencing. PMID: 25684209
  2. MBD7 is required for active DNA demethylation and antisilencing of the genomic loci with high densities of DNA methylation in Arabidopsis. PMID: 25593350
  3. The C-terminal, StkC motif plays an important role in fastening AtMBD7 to its chromosomal, CpG-methylated sites. PMID: 19647732
  4. The characterization of proteins interacting with MBD7, especially PRMT11, is reported. PMID: 17711414
  5. poly-MBD proteins can directly affect chromatin structure by inducing intra- and inter-chromatin compaction via bridging over multiple methylated CpG sites PMID: 18211904

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Subcellular Location
Nucleus. Chromosome.
Tissue Specificity
Expressed in leaves, buds, flowers, stems, siliques, mature seeds and roots.
Database Links

KEGG: ath:AT5G59800

STRING: 3702.AT5G59800.1

UniGene: At.29205

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