MBF1C Antibody

Code CSB-PA881832XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MBF1C Polyclonal antibody
Uniprot No.
Target Names
MBF1C
Alternative Names
MBF1C antibody; At3g24500 antibody; MOB24.13Multiprotein-bridging factor 1c antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MBF1C protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional coactivator that stimulates transcriptional activity by bridging regulatory proteins and TBP, thereby recruiting TBP to promoters occupied by DNA-binding regulators. Involved in the tolerance to heat and osmotic stress by partially activating the ethylene-response signal transduction pathway.
Gene References into Functions
  1. MBF1c functions as a transcriptional regulator which binds DNA and controls the expression of 36 different transcripts during heat stress, including the DRE-binding protein 2A, two heat shock transcription factors, and several zinc finger proteins. PMID: 21457365
  2. possible negative regulator of HSP genes; triple knock-down mutant for MBF1a, MBF1b andtMBF1c sensitive to oxidative stress and osmotic stress PMID: 19783066
  3. constitutive expression of MBF1c enhances the tolerance of transgenic plants to bacterial infection, heat and osmotic stress by perturbing the ethylene-response signal transduction pathway. [Multiprotein bridging factor1 protein] [MBF1c] PMID: 16244138
  4. the existence of a tightly coordinated heat stress-response network, involving trehalose-, SA-, and ethylene-signaling pathways, that is under the control of MBF1c. PMID: 18201973
Subcellular Location
Nucleus, nucleolus. Cytoplasm. Note=PubMed:16283071 shows a localization restricted to the nucleus and not altered by heat or ABA treatment, while PubMed:18201973 describes a transport from the cytoplasm to the nucleus induced by heat stress.
Protein Families
MBF1 family
Tissue Specificity
Expressed in leaves, roots, stems, flowers, siliques and shoots. Not detected in seeds.
Database Links

KEGG: ath:AT3G24500

STRING: 3702.AT3G24500.1

UniGene: At.37513

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