hybC Antibody

Code CSB-PA364785XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) hybC Polyclonal antibody
Uniprot No.
Target Names
hybC
Alternative Names
hybC antibody; b2994 antibody; JW2962Hydrogenase-2 large chain antibody; HYD2 antibody; EC 1.12.99.6 antibody; Membrane-bound hydrogenase 2 large subunit antibody; NiFe hydrogenase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) hybC protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake.
Gene References into Functions
  1. a variant of the hydrogenase 2 large subunit, HybC, containing a fused C-terminal pentapeptide, was isolated. PMID: 22735263
  2. Hydrogen-dependent reduction of benzyl viologen in hydrogenases HyaB, HybC and HybE is dependent on the [Fe-S]-cluster-containing small subunit. PMID: 21717143
  3. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon. PMID: 19917611
Subcellular Location
Cell membrane; Peripheral membrane protein.
Protein Families
[NiFe]/[NiFeSe] hydrogenase large subunit family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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