hyaA Antibody

Code CSB-PA304854XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) hyaA Polyclonal antibody
Uniprot No.
Target Names
hyaA
Alternative Names
hyaA antibody; b0972 antibody; JW0954 antibody; Hydrogenase-1 small chain antibody; HYD1 antibody; EC 1.12.99.6 antibody; Membrane-bound hydrogenase 1 small subunit antibody; NiFe hydrogenase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) hyaA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.
Gene References into Functions
  1. The crystal structure of the Escherichia coli O2-sensitive C19G [NiFe]-hydrogenase-1 variant shows that the mutation results in a novel FeS cluster, proximal to the Ni-Fe active site. PMID: 29888350
  2. Studies indicate that Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, and the arginine/lysine substitution lowers the H2 oxidation rate by nearly 2 orders of magnitude. PMID: 28001048
  3. Crystal structure of dimeric membrane-bound O(2)-tolerant hydrogenase 1 from Escherichia coli in a 2:1 complex with its physiological partner, cytochrome b. PMID: 23260654
  4. These results suggest that the ability to oxidize hydrogen by hydrogenase 1 shows a strong correlation with the rate of carbon flow through glycolysis and provides a direct link between hydrogen, carbon and energy metabolism. PMID: 22241049
Subcellular Location
Cell inner membrane; Single-pass type I membrane protein; Periplasmic side.
Protein Families
[NiFe]/[NiFeSe] hydrogenase small subunit family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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