MCK1 Antibody

Code CSB-PA325186XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MCK1 Polyclonal antibody
Uniprot No.
Target Names
MCK1
Alternative Names
MCK1 antibody; YPK1 antibody; YNL307C antibody; N0392Protein kinase MCK1 antibody; EC 2.7.12.1 antibody; Meiosis and centromere regulatory kinase antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MCK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.
Gene References into Functions
  1. Mck1 identified as a novel positive regulator of inositol de novo synthesis in yeast. Mck1 is required for normal activity of myo-inositol phosphate synthase. PMID: 28817575
  2. Mck1 promotes cell separation and, together with Rim15, controls the cell size after the diauxic shift. PMID: 26103122
  3. Mck1 kinase ensures proper DNA replication, prevents DNA damage, and maintains genome integrity by inhibiting Cdc6. PMID: 25995377
  4. a screen of single-deletion mutants lacking each of the four GSK3 isoforms found that MCK1 is responsible for most of the Elo2 phosphorylation PMID: 24239358
  5. Kns1 and Mck1 are conserved protein kinases that function downstream of TORC1. Efficient repression of ribosome and tRNA synthesis requires Kns1 and Mck1. PMID: 22364741

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Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Database Links

KEGG: sce:YNL307C

STRING: 4932.YNL307C

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