MED33B Antibody

Code CSB-PA194219XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MED33B Polyclonal antibody
Uniprot No.
Target Names
MED33B
Alternative Names
MED33B antibody; MED24B antibody; MED5_1 antibody; MED5B antibody; REF4 antibody; At2g48110 antibody; F11L15.1 antibody; T9J23.26Mediator of RNA polymerase II transcription subunit 33B antibody; Protein REDUCED EPIDERMAL FLUORESCENCE 4 antibody; AtREF4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MED33B protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Involved in the repression of phenylpropanoid biosynthesis. May compete with MED33B for common binding partners or for occupancy in Mediator.
Gene References into Functions
  1. disruption of both REF4 and its paralog, REF4-RELATED 1 (RFR1), results in enhanced expression of multiple phenylpropanoid biosynthetic genes PMID: 22167189
  2. The REF4 locus (At2g48110) was cloned by a combined map- and sequencing-based approach and was found to encode a large integral membrane protein that is unique to plants. PMID: 18430946
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 33 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT2G48110

STRING: 3702.AT2G48110.1

UniGene: At.36290

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