FIB2 Antibody

Datasheet

Code	CSB-PA842466XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FIB2 Polyclonal antibody

Uniprot No.

Q94AH9

Target Names

FIB2

Alternative Names

FIB2 antibody; FLP antibody; MED36_1 antibody; MED36A antibody; At4g25630 antibody; L73G19.10rRNA 2'-O-methyltransferase fibrillarin 2 antibody; AtFib2 antibody; EC 2.1.1.- antibody; Fibrillarin-like protein 2 antibody; Histone-glutamine methyltransferase antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana FIB2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (Probable). Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (Probable). Site specificity is provided by a guide RNA that base pairs with the substrate (Probable). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (Probable). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Acts as negative regulator of expression of immune responsive genes, including pathogenesis-related gene 1 (PR1), and of resistance against bacterial pathogen. Binds to MED19A, a positive regulator of PR1 expression, to repress the activator activity of MED19A. In response to the bacterial pathogen-associated molecular pattern (PAMP) elf18, associates with the long non-coding RNA (lncRNA) ELENA1 (At4g16355), and releases its repression of MED19A. Possesses ribonuclease activity toward rRNA in vitro. Binds phosphoinositides, phospholipids and phosphatidic acid in vitro.

Gene References into Functions

The data indicates that AtFib2 possesses two RNA-binding sites in the central (138-179 amino acids) and C-terminal (225-281 amino acids) parts of the protein, respectively. PMID: 21785141

Subcellular Location

Nucleus, nucleolus.

Protein Families

Methyltransferase superfamily, Fibrillarin family

Tissue Specificity

Expressed in roots and flowers. Expressed in leaves and stems. Expression levels decrease during aging.

Database Links

KEGG: ath:AT4G25630

STRING: 3702.AT4G25630.1

UniGene: At.25589

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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FIB2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background