At1g53240 Antibody

Code CSB-PA222772XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) At1g53240 Polyclonal antibody
Uniprot No.
Target Names
At1g53240
Alternative Names
At1g53240 antibody; F12M16.14 antibody; Malate dehydrogenase 1 antibody; mitochondrial antibody; EC 1.1.1.37 antibody; Mitochondrial MDH1 antibody; mMDH1 antibody; Mitochondrial NAD-dependent malate dehydrogenase 1 antibody; mNAD-MDH 1 antibody; mtNAD-MDH1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana At1g53240 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Required for carbon dioxide and energy partitioning in leaves. May limit photorespiration during the dark phase. Its activity is essential to shuttle reductants out from the mitochondria to support the photorespiratory flux. Can convert 2-oxoglutarate to (S)-2-hydroxyglutarate in vitro.
Gene References into Functions
  1. Results suggest that mitochondrial malate dehydrogenase (mMDH) activity is finely controlled in response to variations in mitochondrial adenine nucleotide balance. PMID: 26946085
  2. a metabolic repair cycle involving mMDH and l-2HGDH is conserved in plant and mammalian mitochondria. [Malate dehydrogenase] [mMDH1] PMID: 26203119
  3. Mitochondrial malate dehydrogenase 1 exhibited a negative correlation of mRNA and protein levels during cold-acclimation and de-acclimation in Arabidopsis. PMID: 25277243
Subcellular Location
Mitochondrion matrix.
Protein Families
LDH/MDH superfamily, MDH type 1 family
Tissue Specificity
Expressed in rosette leaves.
Database Links

KEGG: ath:AT1G53240

STRING: 3702.AT1G53240.1

UniGene: At.23771

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