Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MDN1 Polyclonal antibody

Uniprot No.

Q12019

Target Names

MDN1

Alternative Names

MDN1 antibody; REA1 antibody; YLR106C antibody; L2901 antibody; L8004.13Midasin antibody; Dynein-related AAA-ATPase REA1 antibody; MIDAS-containing protein antibody; Ribosome export/assembly protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MDN1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. At an early stage in 60S maturation, mediates the dissociation of the NOP7 complex (YTM1-ERB1-NOP7) from early pre-60S particles, rendering them competent for export from the nucleolus to the nucleoplasm. Subsequently recruited to the nucleoplasmic particles through interaction with the RIX1 complex. This binding is only possible if the 5S RNP at the central protuberance has undergone the rotation to complete its maturation. After remodeling, removes the ribosome biogenesis factor RSA4 in an ATP hydrolysis-driven step from pre-60S ribosomal subunits, rendering them competent for export from the nucleoplasm to the cytoplasm. Activates the GTPase activity of NOG2, which disengages from the pre-60S particle upon GTP hydrolysis, thus freeing its binding site for the nuclear export factor NMD3.

Gene References into Functions

Release of Nug2 from pre-60S particles requires both its K(+)-dependent GTPase activity and the remodelling ATPase activity of Rea1. PMID: 24240281
Data suggest that Rea1 functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. PMID: 20542003
Rea1 is a dynein-related nuclear AAA-ATPase is involved in late rRNA processing and nuclear export of 60 S subunits PMID: 15528184
Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit. PMID: 19737519

Subcellular Location

Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.

Protein Families

Midasin family

Database Links

KEGG: sce:YLR106C

STRING: 4932.YLR106C

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MDN1 Antibody

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