Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MED12 Polyclonal antibody

Uniprot No.

H3K2Y6

Target Names

MED12

Alternative Names

MED12 antibody; CCT antibody; CRP antibody; MED12_1 antibody; At4g00450 antibody; F5I10.24Mediator of RNA polymerase II transcription subunit 12 antibody; Protein CENTER CITY antibody; Protein CRYPTIC PRECOCIOUS antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MED12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Flowering regulator which suppresses FLC expression, promotes FT and TSF expression and up-regulates SOC1 and FUL mainly in an FT-dependent manner under long-day conditions. Involved in diverse developmental aspects through gene regulation and modulation of the auxin response. Acts closely together with MAB13. Involved in the regulation of embryo patterning and cotyledon organogenesis by transiently repressing a transcriptional program that interferes with this process.

Gene References into Functions

med12 and med13 single mutants exhibit shoot and root phenotypes consistent with altered auxin homeostasis including altered primary root growth, lateral root development, and root hair elongation. PMID: 28780645
GCT and CCT coordinate vegetative and floral transitions by repressing the repressors miR156 and FLC. PMID: 25377553
MED12/CRP is a novel flowering regulator which suppresses FLC expression, promotes FT and TSF expression and up-regulates SOC1 and FUL mainly in an FT-dependent manner under long-day conditions. PMID: 22247249
MED13 and MED12 regulate pattern formation during Arabidopsis embryogenesis by transiently repressing a transcriptional program that interferes with this process. [MED12] PMID: 20023166

Subcellular Location

Nucleus.

Protein Families

Mediator complex subunit 12 family

Tissue Specificity

Ubiquitous. Higher expression in vascular tissue, shoot apex and developing floral organs.

Database Links

KEGG: ath:AT4G00450

UniGene: At.27609

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA183878XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

MED12 Antibody

Product Details

Customer Reviews and Q&A

Target Background