skd Antibody

Code CSB-PA748711XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) skd Polyclonal antibody
Uniprot No.
Target Names
skd
Alternative Names
skd antibody; bli antibody; Med13 antibody; pap antibody; Trap240 antibody; CG9936 antibody; Mediator of RNA polymerase II transcription subunit 13 antibody; Mediator complex subunit 13 antibody; Mediator complex subunit Skuld antibody; Protein blind spot antibody; Protein poils aux pattes antibody; dTRAP240 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) skd protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for leg and eye development and macrochaete specification or differentiation. Negatively regulates sex comb development. Required for activated transcription of the MtnB and MtnD genes.
Gene References into Functions
  1. Data show that both sklud and kohtalo are required to establish normal cell affinity differences at the anterior-posterior and dorsal-ventral compartment boundaries of the wing disc. PMID: 12835386
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 13 family
Database Links

KEGG: dme:Dmel_CG9936

STRING: 7227.FBpp0111743

UniGene: Dm.3624

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