MED14 Antibody

Code CSB-PA928190XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MED14 Polyclonal antibody
Uniprot No.
Target Names
MED14
Alternative Names
MED14 antibody; MED14_1 antibody; SWP antibody; At3g04740 antibody; F7O18.23Mediator of RNA polymerase II transcription subunit 14 antibody; Protein STRUWWELPETER antibody; AtSWP antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MED14 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Involved in defining the duration of cell proliferation.
Gene References into Functions
  1. RNA polymerase II recruitment to CBF-responsive cold-regulated genes requires MED16, MED2, and MED14 subunits. PMID: 24415770
  2. The Arabidopsis Mediator complex subunits MED14 and MED16 differentially regulate defense gene expression in plant immune responses. PMID: 23607369
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 14 family
Tissue Specificity
Expressed in roots, stems, developing embryos, young leaf primordia, shoot apical meristems, inflorescence meristems, tapetum in anthers, ovules and floral organ primordia, but not in mature organs.
Database Links

KEGG: ath:AT3G04740

STRING: 3702.AT3G04740.1

UniGene: At.18555

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