Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAL11 Polyclonal antibody

Uniprot No.

P19659

Target Names

GAL11

Alternative Names

GAL11 antibody; ABE1 antibody; MED15 antibody; RAR3 antibody; SDS4 antibody; SPT13 antibody; YOL051W antibody; Mediator of RNA polymerase II transcription subunit 15 antibody; Autonomous replication regulatory protein 3 antibody; Basal expression activator protein 1 antibody; Defective silencing suppressor protein 4 antibody; Mediator complex subunit 15 antibody; Transcription regulatory protein GAL11 antibody; Ty insertion suppressor protein 13 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAL11 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.

Gene References into Functions

The central activation domain (cAD) of Gcn4 functions through a sequence-specific motif. Optimizing this motif by inserting additional hydrophobic residues creates ADs that bind the Mediator subunit Gal11 via a "fuzzy" protein interface. PMID: 25122681
Phosphorylated sites in the Mediator tail subunit Med15 are required for suppression of stress-induced changes in gene expression under non-stress conditions. PMID: 23135281
Data show that the conserved Gal11 KIX domain contributes to Gal11 function, but found no evidence of specific Gcn4-KIX interaction and conclude that the Gal11 KIX domain does not function by specific interaction with Gcn4. PMID: 20308326
Gcn4 is distinctive in relying on comparable contributions from multiple segments of Gal11 for efficient recruitment of Mediator in vivo PMID: 19940160
GR tau1 directly interacted with the Mediator complex in vivo and in vitro in a Gal11 module-dependent manner, and the Gal11p subunit interacted directly with GR tau1. PMID: 18070925

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Subcellular Location

Nucleus.

Protein Families

Mediator complex subunit 15 family

Database Links

KEGG: sce:YOL051W

STRING: 4932.YOL051W

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GAL11 Antibody

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Target Background