Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MED16 Polyclonal antibody

Uniprot No.

F4JGZ1

Target Names

MED16

Alternative Names

MED16 antibody; MED16_1 antibody; SFR6 antibody; At4g04920 antibody; T1J1.2Mediator of RNA polymerase II transcription subunit 16 antibody; Protein SENSITIVE TO FREEZING 6 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MED16 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Involved in the regulation of the circadian clock, in the control of flowering time, in freezing- and osmotic-stress tolerance and in both salicylic acid- and jasmonate-mediated defense gene expression.

Gene References into Functions

Data show that mutations in MEDIATOR16 (MED16) reduce the number of misregulated genes in cobra (cob-6) mutants and suppress the phenotypes. PMID: 26655738
MED16 regulates resistance to S. sclerotiorum by governing both jasmonic acid/ethylene-mediated and WRKY33-activated defense signaling in Arabidopsis. [MED16] PMID: 26143252
MED16 is involved in the iron deficiency response, and modulates the iron uptake gene expression under iron limitation. PMID: 24889527
Mediator subunit16 (MED16) is an essential positive regulator of systemic acquired resistance (SAR). PMID: 23064320
SFR6 controls defence gene expression mediated by salicylic acid and jasmonate responsive pathways PMID: 22494141
SFR6 acts on the C-box binding factor pathway downstream of CBF translation. PMID: 19067974
sensitive to freezing6 (sfr6) mutation resulted in reduced expression of the clock components CIRCADIAN CLOCK ASSOCIATED1, GIGANTEA, and TIMING OF CAB1. SFR6 Regulates the photoperiodic pathway and circadian clock Gene Expression. [SFR6] PMID: 18614706

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Subcellular Location

Nucleus.

Protein Families

Plant Mediator complex subunit 16 family

Database Links

KEGG: ath:AT4G04920

STRING: 3702.AT4G04920.1

UniGene: At.21855

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MED16 Antibody

Product Details

Customer Reviews and Q&A

Target Background