SRB2 Antibody

Code CSB-PA013653XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SRB2 Polyclonal antibody
Uniprot No.
Target Names
SRB2
Alternative Names
SRB2 antibody; HRS2 antibody; MED20 antibody; YHR041C antibody; Mediator of RNA polymerase II transcription subunit 20 antibody; Hyper-recombination suppressor protein 2 antibody; Mediator complex subunit 20 antibody; Suppressor of RNA polymerase B 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SRB2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
Gene References into Functions
  1. Data show that the temperature sensitive (Ts) growth phenotype of bud13Delta and snu17Delta cells is a consequence of inefficient splicing of MED20 pre-mRNA. This Ts phenotype is partially suppressed by the inactivation of nonsense-mediated mRNA decay (NMD), showing that the growth defects are augmented by the presence of a functional NMD pathway. PMID: 28277935
  2. Gene expression profiling identified a prominent and highly selective role for Med20 in the repression of RP gene transcription under multiple conditions PMID: 18604275
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 20 family
Database Links

KEGG: sce:YHR041C

STRING: 4932.YHR041C

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