Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MED25 Polyclonal antibody

Uniprot No.

Q7XYY2

Target Names

MED25

Alternative Names

MED25 antibody; MED25_1 antibody; PFT1 antibody; At1g25540 antibody; F2J7.4Mediator of RNA polymerase II transcription subunit 25 antibody; AtMED25 antibody; Phytochrome and flowering time 1 protein antibody; Phytochrome and flowering time regulatory protein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana MED25 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Positive regulator of shade avoidance and of jasmonate signaling. Acts in repression of PhyB-mediated light signaling and regulates the expression of FLOWERING LOCUS T (FT) and of CONSTANS (CO).

Gene References into Functions

MED25 links COI1 with HAC1-dependent H3K9 acetylation to activate MYC2-regulated transcription of jasmonic acid-responsive genes. PMID: 28973940
plant density is critical for phase transitions, productivity and root system architecture and reveal a role of Mediator in self-plant recognition. PMID: 28556372
MED25 is necessary for papillae formation on the cell wall surface of leaf trichomes and suggest that the Arabidopsis MED25 mediator component is likely involved in the transcription of a subset of genes that promote papillae deposition in trichomes. PMID: 28889249
The PFT1 short tandem repeat is constrained to its approximate wild-type length by its various functional requirements. PMID: 25116137
PHYTOCHROME AND FLOWERING TIME1 (PFT1), MED25, is degraded by the proteasome and proteasome-mediated PFT1 turnover is coupled to its role in stimulating the transcription of FLOWERING LOCUS T.[MED25] PMID: 22992513
ROS homeostasis is critical throughout the development of root hairs, controlling various processes via PFT1-regulated transcription of genes encoding redox-active enzymes. PMID: 23455023
PFT1 controls root hair growth through redox processes. PMID: 23106228
The MED25 subunit of the Arabidopsis Mediator regulates a wide range of signaling pathways through selectively interacting with specific transcription factors. PMID: 22822206
PFT1 interferes with positively and negatively acting components regulating Arabidopsis light signaling. PMID: 22760208
MED25 is required for the transcription activation ability of MYC2, ERF1 and ORA59. PMID: 22822211
genetic and physiological data suggest that MED25 (PFT1) acts to limit cell and organ growth independently of MED25-mediated phytochrome signaling and the jasmonate pathway PMID: 21903673
Med25 regulates PDF1.2 expression through these three transcription factors. PMID: 21343311
Med25 together with drought response element binding protein 2A function in repression of PhyB-mediated light signaling and thus integrate signals from different regulatory pathways. [Med25] PMID: 21536906
identify the proteins STRUWWELPETER (SWP) and PHYTOCHROME AND FLOWERING TIME 1 (PFT1) as the Med14 and Med25 subunits, respectively PMID: 17560376
Results identify that mediator complex subunit PFT1 is a key regulator of jasmonate signaling pathway within the transcriptional machinery. PMID: 19671879

Hide All

Subcellular Location

Nucleus.

Protein Families

Mediator complex subunit 25 family

Database Links

KEGG: ath:AT1G25540

STRING: 3702.AT1G25540.1

UniGene: At.17319

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MED25 Antibody

Product Details

Customer Reviews and Q&A

Target Background