MED28 Antibody

Code CSB-PA145402XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MED28 Polyclonal antibody
Uniprot No.
Target Names
MED28
Alternative Names
MED28 antibody; MED28_1 antibody; At3g52860 antibody; F8J2.30Mediator of RNA polymerase II transcription subunit 28 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MED28 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
Gene References into Functions
  1. MED32 and MED28 might be functional components in redox signaling/regulation, playing respectively important roles for root development and leaf senescence. [MED28] PMID: 26195288
  2. Data suggest that presence of mediator complex subunits MED10a, MED28, and MED32 or changes in redox state affect DNA-binding capacity of GeBPL (GLABROUS1/GL1 enhancer-binding protein-like) at likely GLABROUS1/GL1 promoter. PMID: 25877331
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 28 family
Database Links

KEGG: ath:AT3G52860

STRING: 3702.AT3G52860.1

UniGene: At.24701

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