melB Antibody

Code CSB-PA360913XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) melB Polyclonal antibody
Uniprot No.
Target Names
melB
Alternative Names
melB antibody; mel-4 antibody; b4120 antibody; JW4081 antibody; Melibiose carrier protein antibody; Melibiose permease antibody; Melibiose transporter antibody; Na+ antibody; Li+)/melibiose symporter antibody; Thiomethylgalactoside permease II antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) melB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Responsible for melibiose and other galactoside transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system).
Gene References into Functions
  1. Lys-377 and Ile-22 of melB are crucial for the substrates binding. PMID: 25971963
  2. Data show that the regulatory protein IIA(Glc) directly binds to melibiose permease (MelB) with dissociation constant value 25 muM. PMID: 25296751
  3. Data describe the three-dimensional structure of melibiose permease at 10 angstrom resolution in the membrane plane with cryo-electron microscopy from two-dimensional crystals. PMID: 16139519
  4. Data suggest that loop 4-5 of melibiose permease contributes to coordinated interactions between ion and sugar binding sites, and participates in an electrogenic conformational transition essential for coupled substrate translocation. PMID: 16822867
  5. MelB-N-ethylmaleimide difference spectra are proportionally less disrupted than the R141C ones PMID: 19527646

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Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
Sodium:galactoside symporter (TC 2.A.2) family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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