Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans met-2 Polyclonal antibody

Uniprot No.

P34544

Target Names

met-2

Alternative Names

met-2 antibody; R05D3.11Histone-lysine N-methyltransferase met-2 antibody; EC 2.1.1.- antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans met-2 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Histone methyltransferase which is required for the mono- and dimethylation of 'Lys-9' of histone H3. This increases the efficiency of set-25-mediated trimethylation of histone H3 'Lys-9'. Involved in the transcriptional repression of lin-3 which is required for the negative regulation of vulval cell fate specification during postembryonic development. Has a role in blocking checkpoint signaling and mediating the transcriptional silencing of meiotic sex chromosome inactivation; a mechanism which enables checkpoint proteins to distinguish between the partnerless male X chromosome and asynapsed chromosomes thereby shielding the lone X from inappropriate activation of an apoptotic program. Operates redundantly with set-25 to position chromatin at the nuclear periphery. Required for small-RNA-induced H3K9 methylation. Together with set-25, protects and stabilizes repeat-rich genomic regions by suppressing transcription-induced replication stress through methylation of H3K9. Together with spr-5, required for transgenerational fertility.

Gene References into Functions

Authors discovered that MET-2 inhibits small RNA inheritance, and, as a consequence, induction of RNAi in met-2 mutants leads to permanent RNAi responses that do not terminate even after more than 30 generations. PMID: 28343968
both histone H3 lysine 9 di- and trimethylation were reduced in Cbr-met-2 mutant germ lines, suggesting that in contrast to C. elegans PMID: 27280692
Study shows that MET-2, a SETDB1 homolog, deposits mono- and dimethyl groups at H3K9, whereas SET-25, a previously uncharacterized histone methyltransferases, trimethylates the same residue. PMID: 22939621
MET-2-dependent H3K9me2 has distinct outputs on the unpaired X chromosome versus asynapsed homologous chromosomes with respect to both checkpoint signaling and transcriptional silencing. PMID: 21909284

Subcellular Location

Nucleus. Chromosome. Cytoplasm.

Protein Families

Class V-like SAM-binding methyltransferase superfamily

Database Links

KEGG: cel:CELE_R05D3.11

STRING: 6239.R05D3.11

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met-2 Antibody

Product Details

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Target Background