metF Antibody

Datasheet

Code	CSB-PA360189XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) metF Polyclonal antibody

Uniprot No.

P0AEZ1

Target Names

metF

Alternative Names

metF antibody; b3941 antibody; JW3913 antibody; 5,10-methylenetetrahydrofolate reductase antibody; EC 1.5.1.20 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) metF protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine.

Gene References into Functions

The invariant aspartate 120 is a critical residue for E. coli methylenetetrahydrofolate reductase, with major roles in folate binding and catalysis, and a minor role in redox modulation. PMID: 15865426
Multiple onformation, which allows aspartic acid 120 to hydrogen bond to the pterin ring in the folate complex but must move to an "open" conformation to allow NADH to bind. PMID: 16114881
Results suggest that Leu may be a suitable replacement for Phe223 in the oxidative half-reaction of E. coli methylenetetrahydrofolate reductase. PMID: 19610625

Protein Families

Methylenetetrahydrofolate reductase family

Database Links

KEGG: ecj:JW3913

STRING: 316385.ECDH10B_4130

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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metF Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background