metI Antibody

Datasheet

Code	CSB-PA339179XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) metI Polyclonal antibody

Uniprot No.

P31547

Target Names

metI

Alternative Names

metI; yaeE; b0198; JW0194; D-methionine transport system permease protein MetI

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) metI protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Part of the binding-protein-dependent transport system for D-methionine and the toxic methionine analog alpha-methyl-methionine. Probably responsible for the translocation of the substrate across the membrane.; (Microbial infection) Probably transports the toxic C-terminal region of CdiA from E.coli strain MHI813 across the inner membrane to the cytoplasm, where CdiA has a toxic effect. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins.

Gene References into Functions

crystal structure of high-affinity MetNI methionine uptake transporter was solved to 3.7 angstrom resolution; methionine binds to dimerized carboxyl-terminal domain & inhibits ATPase activity; findings are consistent with allosteric regulatory mechanism PMID: 18621668

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Binding-protein-dependent transport system permease family, CysTW subfamily

Database Links

KEGG: ecj:JW0194

STRING: 316385.ECDH10B_0179

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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metI Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background